6C5B
Crystal Structure Analysis of LaPhzM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.275, 81.017, 72.164 |
| Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
| Resolution | 71.420 - 1.420 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 3GWZ |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.421 | 1.500 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.441 | |
| Number of reflections | 117067 | |
| <I/σ(I)> | 15.3 | 1.8 |
| Completeness [%] | 97.1 | 98 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium formate, 18 - 22 % w/v PEG 3350 |
