6C4Z

Cross-alpha Amyloid-like Structure alphaAmG - low resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03320
Spacegroup name	P 61 2 2
Unit cell lengths	172.279, 172.279, 153.758
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	149.200 - 3.300
R-factor	0.25081
Rwork	0.250
R-free	0.27669
RMSD bond length	0.006
RMSD bond angle	0.868
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	172.000	3.420
High resolution limit [Å]	3.300	3.300
Number of reflections	38224
<I/σ(I)>	15
Completeness [%]	99.7
Redundancy	16.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	40% MDP, 0.2 M K/Na tartrate