6C4Y

Cross-alpha Amyloid-like Structure alphaAmG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03320
Spacegroup name	P 43 2 2
Unit cell lengths	125.241, 125.241, 119.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	125.240 - 2.500
R-factor	0.23799
Rwork	0.237
R-free	0.25916
RMSD bond length	0.006
RMSD bond angle	0.915
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.000	2.580
High resolution limit [Å]	2.490	2.490
Number of reflections	62540
<I/σ(I)>	19.2
Completeness [%]	100.0
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M ammonium acetate, 0.1 M tri-sodium citrate pH 5.6, 30% (w/v) MPD