6C4W

Structure of the yeast Pichia membranifaciens cytochrome c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	173
Detector technology	AREA DETECTOR
Collection date	1990-12-25
Detector	SDMS
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	27.968, 63.715, 64.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.22197
Rwork	0.216
R-free	0.33046
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ycc
RMSD bond length	0.007
RMSD bond angle	1.674
Data reduction software	DENZO
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.177	0.347
Rmeas	0.223	0.446
Rpim	0.133	0.273
Number of reflections	4586	436
<I/σ(I)>	3.2	1.7
Completeness [%]	95.8	90.4
Redundancy	2.2	2.1
CC(1/2)	0.951	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.25	277	25% PEG 4000, 0.1 M Tris-HCl, pH 8.25