6C4U
Engineered FHA with Myc-pTBD peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 70.180, 72.370, 280.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 140.180 - 2.600 |
| R-factor | 0.2349 |
| Rwork | 0.233 |
| R-free | 0.27910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g6g |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.704 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.180 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmeas | 0.069 | |
| Number of reflections | 47879 | |
| <I/σ(I)> | 16.08 | 1.17 |
| Completeness [%] | 98.4 | 90.4 |
| Redundancy | 6.96 | 5.78 |
| CC(1/2) | 0.990 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.4 M Sodium Malonate |
