6C4N
Pseudopaline dehydrogenase (PaODH) - NADP+ bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.046, 53.634, 96.756 |
| Unit cell angles | 90.00, 99.03, 90.00 |
Refinement procedure
| Resolution | 38.170 - 1.950 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c4l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.777 |
| Number of reflections | 64916 | 4435 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 97.1 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM BisTris pH 5.45, 200 mM ammonium acetate, 26% (w/v) PEG 3350 |
