6C4M
Yersinopine dehydrogenase (YpODH) - NADP+ bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.389, 125.669, 88.012 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.730 - 1.940 |
R-factor | 0.1915 |
Rwork | 0.190 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.076 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.730 | 1.980 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.053 | 0.688 |
Number of reflections | 40471 | 2598 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.2 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.7 M sodium formate pH 6.0, 1.8% ethyl acetate aided by microseeding |