6C4L

Yersinopine dehydrogenase (YpODH) - Apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	97.902, 124.125, 87.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.890 - 2.000
R-factor	0.2021
Rwork	0.200
R-free	0.24530
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.008
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.430	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	1.039
Number of reflections	36673	2550
<I/σ(I)>	8.7
Completeness [%]	99.6
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	3.5 M sodium formation pH 6