6C48

Crystal structure of B-Myb-LIN9-LIN52 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-04-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97741
Spacegroup name	P 1 21 1
Unit cell lengths	60.760, 30.770, 105.350
Unit cell angles	90.00, 99.89, 90.00

Refinement procedure

Resolution	59.857 - 2.320
R-factor	0.2194
Rwork	0.217
R-free	0.26430
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.103
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.860	2.440
High resolution limit [Å]	2.320	2.320
Rmerge	0.127	0.528
Rpim	0.052	0.218
Number of reflections	16249
<I/σ(I)>	9.9
Completeness [%]	94.9
Redundancy	6.6
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5	293	100 mM citric acid 10% PEG 6000