6C3L
Crystal structure of BCL6 BTB domain with compound 15f
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 |
Unit cell lengths | 30.930, 39.589, 55.182 |
Unit cell angles | 83.75, 73.85, 66.96 |
Refinement procedure
Resolution | 30.025 - 1.461 |
R-factor | 0.201385848596 |
Rwork | 0.200 |
R-free | 0.23523 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r29 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.588 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | phenix.refine (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.510 |
High resolution limit [Å] | 1.460 | 1.460 |
Number of reflections | 38467 | |
<I/σ(I)> | 11.4 | |
Completeness [%] | 95.9 | |
Redundancy | 2 | |
CC(1/2) | 0.066 | 0.316 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M sodium acetate pH 4.5, 27% (w/v) PEG-3,350 |