6C36
Mycobacterium smegmatis flap endonuclease mutant D208N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.103, 39.837, 68.313 |
| Unit cell angles | 90.00, 108.72, 90.00 |
Refinement procedure
| Resolution | 38.211 - 1.900 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.20770 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.068 | 0.378 |
| Number of reflections | 48509 | |
| <I/σ(I)> | 10 | 1.5 |
| Completeness [%] | 98.9 | 97.1 |
| Redundancy | 4.2 | 3.3 |
| CC(1/2) | 0.996 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M ammonium acetate, 20% PEG3350 |
