6C35
Mycobacterium smegmatis flap endonuclease mutant D148N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.439, 40.085, 68.419 |
| Unit cell angles | 90.00, 109.11, 90.00 |
Refinement procedure
| Resolution | 38.164 - 1.803 |
| R-factor | 0.1596 |
| Rwork | 0.157 |
| R-free | 0.19310 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.903 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.031 | 0.133 |
| Number of reflections | 55715 | |
| <I/σ(I)> | 22.2 | 3 |
| Completeness [%] | 98.0 | 87.2 |
| Redundancy | 3.9 | 2.8 |
| CC(1/2) | 0.995 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M LiCl, 20% PEG3350 |
