6C33
Mycobacterium smegmatis DNA flap endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.093, 39.828, 68.191 |
| Unit cell angles | 90.00, 108.87, 90.00 |
Refinement procedure
| Resolution | 38.106 - 1.800 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20540 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.887 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.060 | 0.447 |
| Number of reflections | 56545 | |
| <I/σ(I)> | 20.9 | 2 |
| Completeness [%] | 98.8 | 97.4 |
| Redundancy | 5.4 | 3.1 |
| CC(1/2) | 0.997 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM DTT, and 10% glycerol |
