6C33

Mycobacterium smegmatis DNA flap endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	63.093, 39.828, 68.191
Unit cell angles	90.00, 108.87, 90.00

Refinement procedure

Resolution	38.106 - 1.800
R-factor	0.177
Rwork	0.175
R-free	0.20540
RMSD bond length	0.006
RMSD bond angle	0.887
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.800	1.800
Rpim	0.060	0.447
Number of reflections	56545
<I/σ(I)>	20.9	2
Completeness [%]	98.8	97.4
Redundancy	5.4	3.1
CC(1/2)	0.997	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM DTT, and 10% glycerol