6C32
Mycobacterium smegmatis RimJ with AcCoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-16 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.531, 63.531, 227.542 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.067 - 1.898 |
| R-factor | 0.1771 |
| Rwork | 0.175 |
| R-free | 0.20760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.898 | 1.898 |
| Rmerge | 0.855 | |
| Number of reflections | 40630 | 40630 |
| <I/σ(I)> | 18.1 | 3.1 |
| Completeness [%] | 99.4 | 84 |
| Redundancy | 11.5 | |
| CC(1/2) | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium citrate (pH 6.5), 20 % v/v 2-propanol and 20 % w/v polyethylene glycol 4000 |
