6C2F

MBD2 in complex with methylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-07-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	40.510, 40.600, 202.380
Unit cell angles	90.07, 93.11, 119.75

Refinement procedure

Resolution	35.099 - 2.650
R-factor	0.213
Rwork	0.211
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	early version of PDB entry 6C2E model
RMSD bond length	0.010
RMSD bond angle	1.316
Data reduction software	XDS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.100	35.100	2.660
High resolution limit [Å]	2.550	8.830	2.550
Rmerge	0.051	0.037	0.960
Rmeas	0.056	0.041	1.061
Rpim	0.024	0.018	0.448
Total number of observations	198905	4405	24444
Number of reflections	36264	853	4386
<I/σ(I)>	17.3	38.5	1.8
Completeness [%]	99.7	96.9	100
Redundancy	5.5	5.2	5.6
CC(1/2)	0.999	0.998	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20% PEG-3350, 0.2 M potassium acetate