6C1Z

Crystal structure of Apo Caenorhabditis elegans lipid binding protein 8 (LBP-8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	46.904, 41.857, 70.896
Unit cell angles	90.00, 91.14, 90.00

Refinement procedure

Resolution	31.227 - 1.300
R-factor	0.1914
Rwork	0.190
R-free	0.21010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wvm
RMSD bond length	0.008
RMSD bond angle	0.897
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2621)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.350
High resolution limit [Å]	1.300	1.300
Rmeas	0.047	0.666
Rpim	0.018	0.274
Number of reflections	688051	4481
<I/σ(I)>	33.3	1.55
Completeness [%]	96.3	67.9
Redundancy	6.9	4.8
CC(1/2)		0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	2.81 M ammonium sulfate and 0.25 M potassium formate