6C1M

NavAb NormoPP mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.99994
Spacegroup name	I 2 2 2
Unit cell lengths	126.330, 126.244, 191.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.311 - 2.518
R-factor	0.2043
Rwork	0.203
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rvy
RMSD bond length	0.009
RMSD bond angle	1.253
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.310	2.640
High resolution limit [Å]	2.520	2.520
Number of reflections	51039
<I/σ(I)>	18.5
Completeness [%]	99.4
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.8	277	ammonium sulphate, Na-citrate