6C1E
NavAb NormoPP mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.99994 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 126.775, 126.997, 192.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.500 - 2.860 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rvy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 3.000 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Number of reflections | 35059 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277 | ammonium sulphate, Na-citrate |
