6C15
CD1c in complex with phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 84.255, 84.255, 366.833 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.560 - 3.210 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ov6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rpim | 0.047 | 0.477 |
| Number of reflections | 13768 | 1952 |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 43.5 | 45.8 |
| CC(1/2) | 1.000 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10% Dioxane; 1.6 M Ammonium sulfate; 10 mM MES, pH 6.5 |
