6C0Z
Crystal structure of Efga from Methylobacterium extorquens in complex with formaldehyde
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 72.059, 72.063, 104.555 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.315 - 1.830 |
R-factor | 0.2242 |
Rwork | 0.222 |
R-free | 0.26550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a2l |
RMSD bond length | 0.010 |
RMSD bond angle | 0.919 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.830 | 4.970 | 1.830 |
Rmerge | 0.119 | 0.094 | 0.545 |
Total number of observations | 557370 | ||
Number of reflections | 48026 | 2645 | 2293 |
<I/σ(I)> | 7.4 | ||
Completeness [%] | 98.4 | 99.4 | 96.3 |
Redundancy | 11.6 | 11.8 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283.15 | 0.2 M KNO3, 2.2 M AmSO4, 16.6 mM Formaldehyde |