6C08
Zebrafish SLC38A9 with arginine bound in the cytosol open state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 136.613, 82.808, 158.922 |
| Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
| Resolution | 156.498 - 3.170 |
| R-factor | 0.2671 |
| Rwork | 0.267 |
| R-free | 0.28550 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 156.500 | 156.500 | 3.180 |
| High resolution limit [Å] | 3.100 | 14.210 | 3.100 |
| Rmerge | 0.118 | 0.064 | 1.541 |
| Rmeas | 0.127 | 0.069 | 1.642 |
| Rpim | 0.044 | 0.025 | 0.560 |
| Total number of observations | 529570 | 5361 | 37447 |
| Number of reflections | 63753 | 726 | 4391 |
| <I/σ(I)> | 8.3 | 24.3 | 1.1 |
| Completeness [%] | 99.7 | 99.1 | 98.1 |
| Redundancy | 8.3 | 7.4 | 8.5 |
| CC(1/2) | 0.997 | 0.997 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG400, N-(2-Acetamido)iminodiacetic acid pH 7.2, lithium sulfate |
