6BZW

Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the GL precursor of the broadly neutralizing antibody AP33

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03320
Spacegroup name	P 1 21 1
Unit cell lengths	60.356, 84.709, 192.919
Unit cell angles	90.00, 89.96, 90.00

Refinement procedure

Resolution	49.155 - 2.200
R-factor	0.2127
Rwork	0.192
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g6a
RMSD bond length	0.002
RMSD bond angle	0.629
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rpim	0.089	0.417
Number of reflections	93841
<I/σ(I)>	11.85
Completeness [%]	95.4
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (w/v) PEG 4000, 20% (w/v) glycerol, 0.16 M ammonium-sulfate, 0.08 M acetate pH 4.6