6BZV
Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the GL precursor of the broadly neutralizing antibody 19B3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.128, 124.767, 102.407 |
Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
Resolution | 47.357 - 2.654 |
R-factor | 0.2013 |
Rwork | 0.199 |
R-free | 0.24050 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.809 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 49110 | |
<I/σ(I)> | 9.6 | |
Completeness [%] | 95.5 | |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40% 1,2 propanediol, 0.1 M acetate pH 4.5, final pH 5.0 |