6BZM

GFGNFGTS from low-complexity/FG repeat domain of Nup98, residues 116-123

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-03-23
Detector	TVIPS F416 CMOS CAMERA
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	4.790, 18.240, 26.440
Unit cell angles	93.14, 92.73, 97.15

Refinement procedure

Resolution	26.360 - 0.900
R-factor	0.23
Rwork	0.226
R-free	0.26400
Structure solution method	AB INITIO PHASING
RMSD bond length	0.014
RMSD bond angle	1.160
Data scaling software	XSCALE
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.360	26.360	0.920
High resolution limit [Å]	0.900	4.030	0.900
Rmerge	0.289	0.255	0.588
Rmeas	0.313	0.270	0.717
Total number of observations	31674
Number of reflections	5800	63	247
<I/σ(I)>	3.51	8.13	1.12
Completeness [%]	88.0	87.5	47.8
Redundancy	5.461	6.714	2.798
CC(1/2)	0.976	0.972	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	0.1 M CHES, pH 9.5, 10% ethanol