6BZG

Structure of S. cerevisiae Zip2:Spo16 complex, P212121 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	50.249, 96.212, 101.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.809 - 2.130
R-factor	0.2503
Rwork	0.249
R-free	0.27040
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.394
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.190
High resolution limit [Å]	2.130	2.130
Number of reflections	28219	15501
<I/σ(I)>	5.2
Completeness [%]	99.8
Redundancy	6.3
CC(1/2)	0.987	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	100 mM Bis Tris pH 5.5, 200 mM Ammonium sulfate, 15% PEG-3350