6BZF

Structure of S. cerevisiae Zip2:Spo16 complex, C2 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	169.402, 63.589, 199.043
Unit cell angles	90.00, 90.60, 90.00

Refinement procedure

Resolution	57.093 - 2.286
R-factor	0.1984
Rwork	0.196
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bzg
RMSD bond length	0.008
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.330
High resolution limit [Å]	2.286	2.286
Number of reflections	95052	10085
<I/σ(I)>	11.1
Completeness [%]	98.6
Redundancy	3.5
CC(1/2)	0.997	0.328

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.6	293	1.4 M Na-K phosphate pH 6.6