6BZE
Cryo-EM structure of BCL10 CARD filament
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | DIRECT ELECTRON DE-16 (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 4 |
| Number of Particle | 103873 |
| Software | EMAN2,IHRSR,RELION,PHENIX,Leginon |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
