6BZB

Crystal Structure of Glucose-6-phosphate Isomerase from Elizabethkingia anophelis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97876
Spacegroup name	P 21 21 21
Unit cell lengths	69.950, 104.750, 152.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.185 - 1.600
R-factor	0.1492
Rwork	0.149
R-free	0.17680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hjb
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX ((dev_2919))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.185	43.185	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.064	0.026	0.545
Rmeas	0.070	0.028	0.593
Total number of observations	882722
Number of reflections	147843	1802	10823
<I/σ(I)>	17.46	40.97	3.37
Completeness [%]	99.8	97.4	99.9
Redundancy	5.971	5.509	6.151
CC(1/2)	0.999	0.999	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	ElanA.17127.a.B1.PS38341 at 26.1 mg/ml was mixed 1:1 with MCSG1(f1): 0.1 M Bis-Tris:HCl, pH=6.5, 20% (w/v) PEG MME 5000, 20% ethylene glycol cryo. Tray: 295124f1, puck: hay3-9