6BZ7
Thermus thermophilus 70S containing 16S G299A point mutation and near-cognate ASL Leucine in A site.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 210.696, 446.140, 616.004 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 145.572 - 3.680 |
R-factor | 0.2299 |
Rwork | 0.228 |
R-free | 0.27430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v97 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.700 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.12_2829) |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 145.572 | 145.572 | 3.730 |
High resolution limit [Å] | 3.640 | 16.280 | 3.640 |
Rmerge | 0.298 | 0.057 | 1.169 |
Rmeas | 0.323 | 0.061 | 1.302 |
Total number of observations | 4363101 | ||
Number of reflections | 638848 | 7363 | 46922 |
<I/σ(I)> | 6.23 | 25.04 | 1.38 |
Completeness [%] | 99.3 | 95 | 99.3 |
Redundancy | 6.83 | 7.593 | 5.225 |
CC(1/2) | 0.991 | 0.995 | 0.344 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC |