6BZ4
Human IgG1 lacking complement-dependent cytotoxicity: hu3S193 Fc mutant K322A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.324, 79.502, 138.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.300 - 2.400 |
| R-factor | 0.216 |
| Rwork | 0.212 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wi2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.635 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.049 | 0.650 |
| Number of reflections | 21683 | |
| <I/σ(I)> | 22 | 2.4 |
| Completeness [%] | 98.7 | 97.1 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 6000, 0.1 M MES, 25% V/V ETHYLENE GLYCOL |
