6BZ3

Complex structure of FAK FAT domain and DCC P3 motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	129.178, 36.130, 65.429
Unit cell angles	90.00, 104.30, 90.00

Refinement procedure

Resolution	39.889 - 2.497
R-factor	0.2278
Rwork	0.226
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ow6
RMSD bond length	0.006
RMSD bond angle	0.573
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.497	2.497
Rmerge	0.092	0.527
Number of reflections	10318	978
<I/σ(I)>	12.2	2
Completeness [%]	98.7	93.4
Redundancy	3.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M MgCl2, 0.1 M Tris pH8.5, 20% (w/v) PEG8000