6BYY

MEF2 CHIMERA/DNA Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	77
Detector technology	CCD
Collection date	2016-09-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9999
Spacegroup name	P 21 21 2
Unit cell lengths	77.350, 77.760, 106.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.839 - 2.300
R-factor	0.2159
Rwork	0.213
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	PHENIX (dev_2926)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.855	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	29180
<I/σ(I)>	19.81
Completeness [%]	100.0
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M HAc pH 4.7, 150 mM NaCl, 10 mM CaCl2, 5 mM MgCl2 and 28% PEG 400