6BYU

X-ray crystal structure of Escherichia coli RNA polymerase (RpoB-H526Y) and ppApp complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.91790
Spacegroup name	P 21 21 21
Unit cell lengths	186.254, 203.656, 308.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.600
R-factor	0.2449
Rwork	0.244
R-free	0.31130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uaq
RMSD bond length	0.004
RMSD bond angle	0.775
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.660
High resolution limit [Å]	3.600	3.600
Rmerge	0.198
Rmeas	0.217
Rpim	0.087	0.407
Number of reflections	135308	6688
<I/σ(I)>	10
Completeness [%]	100.0	100
Redundancy	6	6
CC(1/2)		0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		300	0.1 M HEPES-HCl (pH 6.7), 0.2 M MgCl2, 30% PEG400, 10 mM DTT