6BYU
X-ray crystal structure of Escherichia coli RNA polymerase (RpoB-H526Y) and ppApp complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 186.254, 203.656, 308.322 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.600 |
| R-factor | 0.2449 |
| Rwork | 0.244 |
| R-free | 0.31130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uaq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.660 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.198 | |
| Rmeas | 0.217 | |
| Rpim | 0.087 | 0.407 |
| Number of reflections | 135308 | 6688 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 6 |
| CC(1/2) | 0.478 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 300 | 0.1 M HEPES-HCl (pH 6.7), 0.2 M MgCl2, 30% PEG400, 10 mM DTT |
