6BYJ

Structure of human 14-3-3 gamma bound to O-GlcNAc peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	122.270, 122.270, 314.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	113.942 - 2.900
R-factor	0.2293
Rwork	0.228
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uzd
RMSD bond length	0.003
RMSD bond angle	0.544
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.942	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.067
Rpim	0.022	0.305
Number of reflections	53876	7734
<I/σ(I)>	18.6	2.5
Completeness [%]	100.0	100
Redundancy	8.8	7
CC(1/2)	0.999	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	29% PEG 4000, 200 mM sodium acetate, 0.1 M Tris pH 8.5