6BYI

Crystal structure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-01
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.459
Spacegroup name	P 1 21 1
Unit cell lengths	68.707, 202.127, 79.095
Unit cell angles	90.00, 110.30, 90.00

Refinement procedure

Resolution	41.970 - 2.200
R-factor	0.171
Rwork	0.169
R-free	0.21150
RMSD bond length	0.004
RMSD bond angle	0.856
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.970	6.540	2.330
High resolution limit [Å]	2.200	4.650	2.200
Number of reflections	97007
<I/σ(I)>	9.08
Completeness [%]	95.0
Redundancy	4.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Ammonium acetate Bis-tris PEG 10000