6BYE
Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.459 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.936, 78.973, 95.585 |
| Unit cell angles | 94.62, 103.42, 111.84 |
Refinement procedure
| Resolution | 38.515 - 2.126 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.23490 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.515 |
| High resolution limit [Å] | 2.120 |
| Number of reflections | 93216 |
| <I/σ(I)> | 14.2 |
| Completeness [%] | 93.1 |
| Redundancy | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Ammonium acetate Bis-tris pH 5.5 PEG 10000 |
