6BYC
Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.459 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.502, 68.575, 79.021 |
| Unit cell angles | 90.00, 95.16, 90.00 |
Refinement procedure
| Resolution | 19.978 - 1.897 |
| R-factor | 0.1766 |
| Rwork | 0.174 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2je8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.263 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.978 | 5.640 | 2.010 |
| High resolution limit [Å] | 1.897 | 4.010 | 1.900 |
| Number of reflections | 71912 | ||
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 94.9 | ||
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Ammonium acetate Bis-Tris pH 5.5 PEG 10000 |
