6BY9
Crystal structure of EHMT1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 53.681, 53.681, 204.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.490 - 2.300 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.7.17) |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.490 | 46.490 | 2.380 |
High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
Rmerge | 0.061 | 0.037 | 1.251 |
Rmeas | 0.066 | 0.040 | 1.336 |
Rpim | 0.024 | 0.016 | 0.463 |
Total number of observations | 127072 | 2100 | 12370 |
Number of reflections | 16043 | 345 | 1531 |
<I/σ(I)> | 15.6 | 42.5 | 1.9 |
Completeness [%] | 99.8 | 99.7 | 99.3 |
Redundancy | 7.9 | 6.1 | 8.1 |
CC(1/2) | 0.998 | 0.997 | 0.938 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M BIS-TRIS pH6.5,3.0M NaCl |