6BXW
Crystal structure of Toxoplasma gondii Mitochondrial Association Factor 1 B (MAF1B) in complex with ADPribose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.470, 62.360, 89.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.556 - 1.650 |
R-factor | 0.1969 |
Rwork | 0.196 |
R-free | 0.21910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bxr |
RMSD bond length | 0.004 |
RMSD bond angle | 0.856 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.556 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.094 | 0.553 |
Number of reflections | 29065 | |
<I/σ(I)> | 7.6 | 2.2 |
Completeness [%] | 93.4 | 97.4 |
Redundancy | 3.9 | 3.8 |
CC(1/2) | 0.991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M ammonium sulfate, 20% PEG3350 |