6BXV

SYSSYGQS from low-complexity domain of FUS, residues 54-61

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	49.580, 4.785, 21.812
Unit cell angles	90.00, 95.46, 90.00

Refinement procedure

Resolution	24.680 - 1.100
R-factor	0.1378
Rwork	0.137
R-free	0.14770
Structure solution method	AB INITIO PHASING
RMSD bond length	0.004
RMSD bond angle	0.755
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.140
High resolution limit [Å]	1.100	2.370	1.100
Rmerge	0.139	0.073	0.514
Rmeas	0.147	0.077	0.583
Rpim	0.046	0.024	0.268
Total number of observations	18606
Number of reflections	2168	232	196
<I/σ(I)>	6.9
Completeness [%]	90.9	86.9	82
Redundancy	8.6	8.1	4.2
CC(1/2)		0.991	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.5M ammonium tartrate dibasic pH 7.0