6BXS
Crystal structure of Toxoplasma gondii Mitochondrial Association Factor 1 A (MAF1A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.890, 49.670, 114.750 |
| Unit cell angles | 90.00, 96.09, 90.00 |
Refinement procedure
| Resolution | 48.860 - 2.100 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bxr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.686 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.860 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.444 |
| Number of reflections | 51862 | |
| <I/σ(I)> | 11.5 | 3 |
| Completeness [%] | 98.5 | 92.4 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.9 M ammonium sulfate, 0.1M Hepes pH 7.0, 0.5% PEG8000, 3% 2- methyl- 2,4- pentanediol |
