6BXR
Crystal structure of Toxoplasma gondii Mitochondrial Association Factor 1 B (MAF1B)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-29 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9195 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.090, 62.110, 89.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.605 - 1.600 |
R-factor | 0.1751 |
Rwork | 0.173 |
R-free | 0.21320 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.819 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXS |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.605 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.475 | |
Number of reflections | 33786 | |
<I/σ(I)> | 12.7 | 3.5 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 8.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 0.2 M ammonium sulfate, 20% PEG3350 |