6BXE
Crystal structure of Variable Lymphocyte Receptor 9 (VLR9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 61 |
| Unit cell lengths | 75.511, 75.511, 99.307 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.291 - 1.651 |
| R-factor | 0.1538 |
| Rwork | 0.153 |
| R-free | 0.17350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3twi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.090 | |
| Rpim | 0.020 | 0.280 |
| Number of reflections | 38111 | 1669 |
| <I/σ(I)> | 38.2 | 1.9 |
| Completeness [%] | 98.9 | 86.6 |
| Redundancy | 18.3 | 10.7 |
| CC(1/2) | 0.950 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2.0 M (NH4)2SO4, 0.1 M citrate pH 5.5 |
