6BXE

Crystal structure of Variable Lymphocyte Receptor 9 (VLR9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 61
Unit cell lengths	75.511, 75.511, 99.307
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.291 - 1.651
R-factor	0.1538
Rwork	0.153
R-free	0.17350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3twi
RMSD bond length	0.007
RMSD bond angle	0.915
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.090
Rpim	0.020	0.280
Number of reflections	38111	1669
<I/σ(I)>	38.2	1.9
Completeness [%]	98.9	86.6
Redundancy	18.3	10.7
CC(1/2)	0.950	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2.0 M (NH4)2SO4, 0.1 M citrate pH 5.5