EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | DIRECT ELECTRON DE-20 (5k x 3k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.79 |
| Number of Particle | 8404 |
| Software | Auto3DEM,Coot,UCSF Chimera |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
