6BWM

Crystal structure of the TRPV2 ion channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	92.028, 122.356, 187.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.851 - 3.900
R-factor	0.2468
Rwork	0.244
R-free	0.29270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5an8
RMSD bond length	0.003
RMSD bond angle	0.657
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.851	4.270
High resolution limit [Å]	3.900	3.900
Rpim	0.190	1.190
Number of reflections	19867
<I/σ(I)>	8.8
Completeness [%]	99.8
Redundancy	17.5
CC(1/2)	0.990	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	PEG400, Calcium chloride