6BWB

Hendra virus W protein C-terminus in complex with Importin alpha 3 crystal form 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	48.160, 58.990, 169.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.495 - 2.300
R-factor	0.1798
Rwork	0.178
R-free	0.21840
RMSD bond length	0.006
RMSD bond angle	0.710
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.495	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.032	0.077
Number of reflections	22252	2139
<I/σ(I)>	17.1	8.8
Completeness [%]	100.0	100
Redundancy	12.9	13.1
CC(1/2)	0.997	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M sodium chloride, 0.1M MES pH6.5, 10% w/v PEG 4000