6BW9

Hendra virus W protein C-terminus in complex with Importin alpha 3 crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	47.430, 65.740, 74.190
Unit cell angles	90.00, 99.76, 90.00

Refinement procedure

Resolution	19.578 - 1.600
R-factor	0.1725
Rwork	0.171
R-free	0.19630
RMSD bond length	0.015
RMSD bond angle	1.216
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.840	1.630
High resolution limit [Å]	1.600	1.600
Rpim	0.061	0.174
Number of reflections	58984	2917
<I/σ(I)>	7.1	3.3
Completeness [%]	99.4	99.6
Redundancy	4.3	4.4
CC(1/2)	0.989	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M sodium citrate pH 5.0, 20% w/v PEG 2000