6BW1

Hendra virus W protein C-terminus in complex with Importin alpha 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-08
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	79.040, 89.270, 100.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.760 - 2.200
R-factor	0.1925
Rwork	0.192
R-free	0.21220
RMSD bond length	0.003
RMSD bond angle	0.619
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.760	2.280
High resolution limit [Å]	2.200	2.200
Rpim	0.029	0.147
Number of reflections	36721	3139
<I/σ(I)>	18.5	5.4
Completeness [%]	99.8	100
Redundancy	5.7	5.8
CC(1/2)	0.999	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.01M DTT, 0.1M sodium HEPES pH7, 0.7M sodium citrate