6BVT
Importin alpha 1 in cargo free state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.516, 90.007, 100.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.540 - 2.500 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fc8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.586 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.540 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.061 | 0.365 |
| Number of reflections | 24805 | 2822 |
| <I/σ(I)> | 10.2 | 2.9 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 6.2 | 6.2 |
| CC(1/2) | 0.989 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.01M DTT, 0.1M sodium HEPES, 1M Ammonium Sulfate |
