6BV8
Structure of proteinaceous RNase P 1 (PRORP1) from A. thaliana complexed with Mn after 3-hour soak with juglone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.677, 111.332, 139.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.990 - 2.100 |
| R-factor | 0.20222 |
| Rwork | 0.200 |
| R-free | 0.23608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g23 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.312 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.990 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.636 |
| Number of reflections | 38500 | |
| <I/σ(I)> | 13.4 | 1.6 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 18% PEG3350, 0.1 M sodium citrate tribasic, pH 5.5 |
